KMID : 0624620080410090664
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BMB Reports 2008 Volume.41 No. 9 p.664 ~ p.669
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Expression and localization of the spermatogenesis-related gene, Znf230, in mouse testis and spermatozoa during postnatal development
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Song Hong-Xia
Su Dan Lu Pan Yang Jiyun Zhang Wei Yang Yuan Liu Yun-Qiang Zhang Sizhong
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Abstract
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Znf230, the mouse homologue of the human spermatogenesis-related gene, ZNF230, has been cloned by rapid amplification of cDNA ends (RACE). This gene is expressed predominantly in testis, but its expression in different testicular cells and spermatogenic stages has not been previously analyzed in detail. In the present study, the cellular localization of the Znf230 protein in mouse testis and epididymal spermatozoa was determined by RT-PCR, immunoblotting, immunohistochemistry and immunofluorescence. It is primarily expressed in the nuclei of spermatogonia and subsequently in the acrosome system and the entire tail of developing spermatids and spermatozoa. The results indicate that Znf230 may play an important role in mouse spermatogenesis, including spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization.
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KEYWORD
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Expression, Immunofluorescence, Immunohistochemistry, Localization, Spermatogenesis
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